Differential Abundance (DESeq2)

Select groups from group_id then select/deselect samples as needed before running DESeq2.

Select the treatment group to test.

Select the control group.

1a. Min Abundance Filter: 0

1b. Min Rel. Abund (%): 0.01

1c. Min Prevalence (%): 5

2. Exclude Taxonomy

Separate multiple terms with commas (e.g., Chloroplast,Mitochondria).

Batch Correction Method

Log₂(x + 1) Transformation

เมื่อเปิดใช้ → จะใช้ Welch's T-test แทน DESeq2

Processing Order

① Filter ASVs② Log₂③ DESeq2 (NB model)

⚪ Raw counts → DESeq2 (Negative Binomial model + Benjamini-Hochberg FDR, แนะนำสำหรับ microbiome count data)