Rarefaction Curves

Check if sequencing depth is sufficient using the Hurlbert (1971) analytical rarefaction formula.

E(S_n) = Σ [1 − C(N−N_i, n) / C(N, n)] — expected ASVs at subsample depth n

1. Min Relative Abundance (%): 0.005%

2. Min Prevalence (%): 5%

3. Exclude Taxonomy